Escherichia Coli Bl21: A Useful Biocatalyst for the Synthesis of Purine Nucleosides
نویسندگان
چکیده
منابع مشابه
Stimulatory effect of low ATP pools on transport of purine nucleosides in cells of Escherichia coli.
Cells of Escherichia coli contain two nucleoside-transport systems. Energy-starved cells of a strain containing only one of these systems and, in addition, carrying a mutation in the Ca2+- and Mg2+-dependent ATPase (ATP phosphohydrolase 3.6.1.3) are still able to transport nucleosides. The rate is only slightly lower than the rate measured in unstarved cells. Freshly harvested uncA cells transp...
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Although many tracer studies on the purine metabolism of higher organisms have been reported, there are only preliminary data (1, 2) on the purine metabolism of Escherichiu coli, strain B, the host of the “T series” of bacteriophages. The work presented in this paper was undertaken concomitantly with an investigation of the purine metabolism of the virusinfected cell (3). The study of the purin...
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In spite of the fact that commercial bromelain supplements are available in the market, to date, none of them are produced and formulated from recombinant forms. They are extracted and purified (often partially) from the stem and fruit of pineapple (Ananas comosus). This makes the production of bromelain very difficult, less reliable, often contaminated and expensive. In this study, a recombina...
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Escherichia coli BL21(DE3) has long served as a model organism for scientific research, as well as a workhorse for biotechnology. Here we present the most current genome annotation of E. coli BL21(DE3) based on the transcriptome structure of the strain that was determined for the first time. The genome was annotated using multiple automated pipelines and compared to the current genome annotatio...
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The thermostable properties of Taq DNA polymerase from Thermus aquaticus have contributed greatly to the yield, specificity, automation, and utility of the polymerase chain reaction method for amplifying DNA. Taq polymerase is widely used enzyme for DNA amplification in PCR techniques and highly applicable in molecular biology and biotechnology. In this study the Taq gene was amplified from the...
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ژورنال
عنوان ژورنال: Molecules
سال: 2000
ISSN: 1420-3049
DOI: 10.3390/50300535